EFFECT OF GLUCOSE AND FRUCTOSE ON PRODUCTION POLYHYDROXYALKANOATES BY Ralstonia eutropha JMP 134 AS BATCH FERMENTATION

Abstract

Polyhydroxyalknoate (PHA) is a bioplastic from the group of polyester with physicochemical properties similar to polypropylene plastic from petroleum. This research aims to study the effect of glucose and fructose on PHA fermentation process performed by using Ralstonia eutropha JMP 134 in a batch bioreactor. Dynamics of PHA production  from carbon sources glucose or fructose, as well as the influence of volatile fatty acids as a prekursor were studied in this research.  Fermentation operating condition using bioreactor 10 L maintained at a temperature of 30^oC and pH of 7.  The concentration of carbon source used was 40 g/L, and after 20^th hours of  fermentation added volatile fatty acids (VFAs) that serves as a prekursor in the production of PHA. Aerobic fermentation of Ralstonia eutropha JMP 134 with the substrate glucose and fructose using synthetic VFAs produce PHA content of each were 0.21 and 0.33 g PHA/g dry cell weight (DCW). The percentage composition PHA of glucose or fructose with synthetic ALV are 8.71% or 11.78% HV, respectively. So Ralstonia eutropha JMP 134 fermentation using fructose substrate provides a higher PHA content than using glucose substrate.